# Trypsin

> Bulk Trypsin EC 3.4.21.4 supply for B2B protein-processing applications. Request a free sample or quote for USP 2,500+ U/mg enzyme powder used in cell culture, bioprocessing, proteomics, insulin production, and wound care.

## About
Trypsin is available from enzymehero.com — an industrial enzyme supplier.
Contact: hello@mail.enzymehero.com

## Applications
- Cell Culture & Passaging: Standard cell dissociation enzyme for adherent cell cultures—detaches cells from culture vessels for passaging.
- Vaccine Production: Cell harvesting in vaccine manufacturing—trypsinization of Vero, MDCK, and HEK293 cells at production scale.
- Proteomics & Mass Spec: In-gel and in-solution protein digestion for bottom-up proteomics—the gold standard for LC-MS/MS sample prep.
- Insulin Production: Proteolytic processing of proinsulin to active insulin—critical step in recombinant insulin manufacturing.
- Wound Debridement: Pharmaceutical-grade trypsin for enzymatic wound debridement—removes necrotic tissue in chronic wound care.
- Recombinant (Animal-Free): TrypLE and corn-derived recombinant trypsin—animal-origin-free alternative for GMP cell therapy manufacturing.

## Specifications
- Enzyme Name: Trypsin (EC 3.4.21.4)
- Activity: USP: 2,500+ USP units/mg
- pH Range: 7.0 – 9.0
- Temperature: 37°C
- Appearance: White to off-white lyophilized powder
- Source: Bovine/Porcine pancreas, Recombinant (corn)
- Shelf Life: 36 months (-20°C), 12 months (2-8°C)
- Packaging: 1 g / 5 g / 100 g / 1 kg
- MOQ: 1 g (research) / 100 g (bulk)
- Certifications: USP/NF, cGMP, Animal-Free available, Irradiated available

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