High-activity Trypsin for consistent proteolytic performance in cell culture, bioprocessing, and analytical workflows. Works best within recommended pH and temperature ranges. Activity 2,500+ USP units/mg. Optimal pH 7.0–9.0 and 37°C. White to off-white lyophilized powder. Shelf life up to 36 months at -20°C. Packaging 1 g / 5 g / 100 g / 1 kg. MOQ 1 g (research) / 100 g (bulk).
Quality USP/NF & cGMP
Trypsin (EC 3.4.21.4)
USP: 2,500+ USP units/mg
7.0 – 9.0
37°C
White to off-white lyophilized powder
Bovine/Porcine pancreas, Recombinant (corn)
36 months (-20°C), 12 months (2-8°C)
1 g / 5 g / 100 g / 1 kg
1 g (research) / 100 g (bulk)
USP/NF, cGMP, Animal-Free available, Irradiated available
Trypsin is a serine protease used to cleave peptide bonds and enable controlled protein processing. It is widely applied in cell dissociation, biomanufacturing, proteomics digestion, and pharmaceutical enzymatic debridement when operated within validated conditions.
Standard cell dissociation enzyme for adherent cell cultures—detaches cells from culture vessels for passaging.
Cell harvesting in vaccine manufacturing—trypsinization of Vero, MDCK, and HEK293 cells at production scale.
In-gel and in-solution protein digestion for bottom-up proteomics—the gold standard for LC-MS/MS sample prep.
Proteolytic processing of proinsulin to active insulin—critical step in recombinant insulin manufacturing.
Pharmaceutical-grade trypsin for enzymatic wound debridement—removes necrotic tissue in chronic wound care.
TrypLE and corn-derived recombinant trypsin—animal-origin-free alternative for GMP cell therapy manufacturing.
Trypsin is commonly used for 🧫 cell dissociation/passaging, 💉 vaccine cell harvesting, 🧬 proteomics digestion for LC-MS/MS, 💊 proinsulin processing in insulin manufacturing, and 🩹 enzymatic wound debridement.
For best performance, use Trypsin at pH 7.0–9.0 and 37°C, matching typical cell culture and proteolysis conditions.
This indicates the measured enzyme activity per gram based on USP testing. Higher activity generally supports faster cleavage at the same mass; final dosing should be confirmed in your specific application.
Yes. Animal-free options are available, including recombinant (corn) trypsin and related alternatives suitable for GMP cell therapy manufacturing.
Shelf life is 36 months at -20°C and 12 months at 2–8°C. Keep the lyophilized powder sealed and follow the COA/TDS storage guidance provided with your order.
Certifications & Standards
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