Trypsin Controlled Cleavage for Cell Harvesting, Proteomics and Bioprocessing (EC 3.4.21.4)
Consistent high-activity Trypsin for reliable proteolysis across cell culture, biomanufacturing, and analytical workflows. Activity ≥2,500 USP units/mg, optimal pH 7.0–9.0 at 37°C. White to off-white lyophilized powder with a shelf life of 36 months (-20°C) or 12 months (2–8°C). Pack sizes 1 g / 5 g / 100 g / 1 kg. MOQ 1 g (research) / 100 g (bulk). For manufacturers, research buyers and distributors: send the product, quantity and destination country for a quote.
Quality USP/NF & cGMP
Product Specifications
01 Enzyme Name
Trypsin (EC 3.4.21.4)
02 Activity
USP: 2,500+ USP units/mg
03 pH Range
7.0 – 9.0
04 Temperature
37°C
05 Appearance
White to off-white lyophilized powder
06 Source
Bovine/Porcine pancreas, Recombinant (corn)
07 Shelf Life
36 months (-20°C), 12 months (2-8°C)
08 Packaging
1 g / 5 g / 100 g / 1 kg
09 MOQ
1 g (research) / 100 g (bulk)
10 Certifications
USP/NF, cGMP, Animal-Free available, Irradiated available
Applications
Trypsin is a serine protease used for precise peptide-bond cleavage in validated industrial and laboratory workflows. Commonly applied in cell dissociation, vaccine manufacturing, proteomics digestion, insulin processing, and topical pharmaceutical preparations within defined pH and temperature ranges.
Cell Culture & Passaging
Standard cell dissociation enzyme for adherent cell cultures—detaches cells from culture vessels for passaging.
Learn more →Vaccine Production
Cell harvesting in vaccine manufacturing—trypsinization of Vero, MDCK, and HEK293 cells at production scale.
Proteomics & Mass Spec
In-gel and in-solution protein digestion for bottom-up proteomics—the gold standard for LC-MS/MS sample prep.
Insulin Production
Proteolytic processing of proinsulin to active insulin—critical step in recombinant insulin manufacturing.
Topical Pharmaceutical Formulation
Trypsin as a protease ingredient for makers of topical pharmaceutical preparations; grade, source and documents are confirmed with each quote.
Recombinant (Animal-Free)
TrypLE and corn-derived recombinant trypsin—animal-origin-free alternative for GMP cell therapy manufacturing.
Learn About Trypsin
Industry guides covering process windows, supplier qualification, dosage, and applications — written for procurement and process engineers.
Enzyme Trypsin Specification Checklist for Cell Culture Buyers
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Enzyme Trypsin Is A Part Of Which Juice
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How to Use Enzymes Trypsin in Cell Culture Formulations
Formulate trypsin enzyme for cell culture with pH, temperature, dose, QC, COA/TD…
Juice That Contains Trypsin Enzyme
B2B guide to trypsin enzyme use in juice formulations: pH, temperature, dosage, …
Trypsin Enzyme Mediated Hydrolysis for Protein Hydrolysate Producers
For protein hydrolysate producers: where trypsin fits in a controlled enzymatic …
Why Source From Us
Frequently Asked Questions
Q1 What is Trypsin (EC 3.4.21.4) primarily used for?
Trypsin is commonly used for 🧫 cell dissociation/passaging, 💉 vaccine cell harvesting, 🧬 proteomics digestion for LC-MS/MS, 💊 proinsulin processing in insulin manufacturing, and 🩹 topical pharmaceutical preparations.
Q2 What are the recommended pH and temperature for Trypsin activity?
For optimal performance, use Trypsin at pH 7.0–9.0 and 37°C, matching typical cell culture and proteolysis conditions.
Q3 How should I interpret “USP: 2,500+ USP units/mg” on the label?
This reflects enzyme activity per milligram based on USP testing; higher activity can enable faster cleavage at equivalent dosage, pending application-specific validation.
Q4 Do you offer animal-free Trypsin options?
Yes. Animal-free options are available, including recombinant (corn) trypsin and related alternatives suitable for GMP cell therapy manufacturing.
Q5 What storage conditions maximize shelf life?
Shelf life is 36 months at -20°C and 12 months at 2–8°C. Keep the lyophilized powder sealed and follow COA/TDS storage guidance provided with your shipment.
Q6 What should a quote request include?
The product (grade and source: bovine, porcine or recombinant corn), the quantity, and the destination country, plus your application. Pack sizes are 1 g / 5 g / 100 g / 1 kg; MOQ is 1 g (research) / 100 g (bulk). Prices are quoted per order.
Certifications & Standards
Get a Free Sample & Quote
Send the product (grade and source), quantity and destination country. Tell us your application and target volume. We’ll send a free sample, COA, and a competitive quote within 24 hours.
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